Outline
- Abstract
- Keywords
- Introduction
- Materials and Methods
- Dna Extraction
- Data Analysis
- Results and Discussion
- References
رئوس مطالب
- چکیده
- کلیدواژه ها
- مقدمه
- مواد و روش ها
- استخراج DNA
- تحلیل های داده ها
- نتایج و مباحث
Abstract
Genetic relationships between eighteen medicinal were assayed with Random Amplified Polymorphic DNA (RAPD) markers, which distinguish individuals, as well as reflecting the inherent variation and interrelationships among the medicinal plants. 13 decamer RAPD primers were used in the present study. Over 77.23 reproducible bands were generated by RAPD primers, out of which, 77.23 polymorphic bands were identified, conferring 97.6% polymorphism. All the primers produced typical banding in each of the medicinal plants, suggesting the applicability of this test in medicinal plant identification. Most of the individuals of the test exhibited to have unique molecular genotype. Population genetics structure analysis of these species further revealed high genetic differentiation coefficients (Gst), the heterozygosity among populations (Ht) showed with the low gene flow (Nm) when the 1st cluster was paired with other medicinal plants On the basis of these parameters and the results of cluster analysis. It is concluded that three species can be considered as a separate group of medicinal plant, whereas the other four clusters may be grouped separately. A dendrogram was constructed using Euclidean distance methods. Based on the number of bands the of the medicinal plants were grouped to form 1-4 clusters.
Keywords: Diversity - Genetic - Medicinal - plants - RAPDConclusions
The genomic DNA of 18 medicinal plants viz , Hemigraphis colorata, Marjorana hortensis, Artemisia vulgaris, Artemisia pallens, Ocimum sanctum, Ocimum basilicum, Ocimum hratissimum, Mentha piparita, Mentha citrate, Mentha spicata, Acorus calamus, Centella asiatica, Bacopa moninierii, Piper longum, Piper nigrum, Clitoria ternatea, Aloe vera, Stevia rebaudia were amplified with oligonucleotides primers OPD-13 revealed total of 172 RAPD bands, however, the medicinal plants such as Artemisia vulgaris, Artemisia pallen Mentha piparita, Mentha citrate Bacopa moninierii and Stevia rebaudia have showed 9 RAPD bands respectively. Further, oligonucleotides primers OPD -13 have showed 8-9 RAPD bands in each medicinal plant. The number and size of the amplification products varied depending up on the sequence of random primers and medicinal plants. The size of the amplified products ranged from 300-5000bp with an average of 9.5 bands per primer. The amplified products were generated by primers showing polymorphic bands Fig 1 and 14. The genomic were amplified with OPD-07 revealed a total of 104 RAPD bands with an average of 6 bands per primer with the primer sequence of TTGGCACGGG..All the 18 medicinal plants exhibited 10 RAPD bands respectively. Whereas the some of the medicinal plants such as Artemisia vulgaris Mentha citrate and Piper longum have expressed more than 10 RAPD bands. Shown in Fig 2 and 15. The primer OPL-11 shown 63 RAPD bands. An Out of these 63 RAPD bands of the medicinal plants plant such as Acorus calamu is revealed 10 RAPD bands. Whereas other medicinal have revealed 7-8 RAPD bands respectively as shown in fig 3 and 16. The genomic DNA of medicinal plants were amplified with the primer sequence OPO 08 revealed a total of 89 RAPD bands. Out of these 89 RA PD bands indicating both monomorphic and polymorphic character with an average of 4.9 per primer.