Outline

  • Abstract
  • Keywords
  • 1. Introduction
  • 2. Materials and Methods
  • 2.1. Hollow-Core Optical Fiber As a Conduit for Plasmas
  • 2.2. Fabrication of the Hollow-Core Optical Fiber-Based Plasma Jet Devices
  • 2.3. System for Atmospheric Pressure Microplasma Jets
  • 2.4. Cell Preparation and Cell Treatments by Microplasma Jets for Annexin V-Fitc Assay
  • 2.5. Cell Preparation and Cell Treatments by Microplasma Jets for Tunel Assay
  • 3. Results and Discussion
  • 3.1. Plasma Plumes from Hollow-Core Optical Fiber Based Plasma Jet Devices
  • 3.2. Apoptotic Analysis of Microplasma Treatments Using Annexin V-Fitc
  • 3.3. Apoptotic Analysis of Microplasma Treatments Using Tunel Assay
  • 4. Conclusions
  • Acknowledgments
  • Appendix A. Supplementary Data
  • References

رئوس مطالب

  • چکیده
  • 1. مقدمه
  • 2. مواد و روش ها
  • 2.1 فیبر نوری توخالی به عنوان یک مجرا برای پلاسما
  • 2.2 تولید دستگاه های جت پلاسمای مبتنی بر فیبر نوری توخالی
  • 2.3 سیستم برای جت های میکرو پلاسمای فشار اتمسفری
  • 2.4 آماده سازی سلول و درمان سلول توسط جت های میکرو پلاسما برای سنجش انکسین V-FITC
  • 2.5 آماده سازی سلول و درمان سلول توسط جت های میکرو پلاسما برای سنجش TUNEL
  • 3. بحث و نتیجه گیری
  • 3.1 دودهای پلاسما از دستگاه های جت پلاسما مبتنی بر فیبر نوری توخالی
  • 3.2 تحلیل کشندگی درمان های میکرو پلاسما با استفاده از انکسین V-FITV
  • 3.3 تحلیل کشندگی درمان های میکرو پلاسما با استفاده از سنجش TUNEL
  • 4. نتیجه گیری

Abstract

Atmospheric pressure plasmas have been used as a therapy for cancer. However, the fairly large size and rigidity of present plasma-delivery systems obstructs the precise treatment of tumors in harder-to-reach internal organs such as the lungs, pancreas, and duodenum. In order to improve the targeted delivery of plasmas a highly flexible microplasma jet device is fabricated using a hollow-core optical fiber with an inner diameter of either 15 μm, 55 μm, or 200 μm. Described herein, based on this device, are results on lung carcinoma therapy using a microplasma cancer endoscope. Despite the small inner diameter and the low gas flow rate, the generated plasma jets are shown to be sufficiently effective to induce apoptosis, but not necrosis, in both cultured mouse lung carcinoma and fibroblast cells. Further, the lung carcinoma cells were found to be more sensitive to plasma treatment than the fibroblast cells based on the overall plasma dose conditions. This work enables directed cancer therapies using on highly flexible and precise hollow optical fiber-based plasma device and offers enhancements to microplasma cancer endoscopy using an improved method of plasma targeting and delivery.

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